Wide-field fluorescence microscopy

Regular wide-field fluorescence imaging is possible with the Zeiss Axio Observer Z1 inverted microscope. The fully motorized system is well suited for cell imaging and features Definite Focus to eliminate Z drift during long-term observations. In addition, a heated stage is available for temperature control of live specimens.

​

Currently one filter set is available (Zeiss 62 HE), whichis compatible with a wide range of dyes including for example DAPI, Alexa 350/405/430/488/568/594, Calcein, Cy 2, FITC, fluorescein, mCherry, MitoTracker Green, mPlum, mStrawberry, Nile Red, SYBR Green, and SYPRO Ruby.

​

Available objectives on the Zeiss Axio Observer Z1 are:

• ​Plan-Neofluar 10x/0.3

• Plan-Apochromat 20x/0.8

• Plan-Apochromat 63x/1.4

Confocal and multi-photon imaging

For confocal and multi-photon imaging a Zeiss LSM 780 inverted confocal microscope with a tunable Coherent Chameleon laser is available. Available wavelengths are: 458, 488, 514, 543, 594 and a tunable laser from 680-1080 nm.

​

Please note that no dedicated UV laser is available. However, it is possible to utilize two-photon excitation at 700-820 nm (Em 455,478 nm) to visualize DAPI stains. For other dyes empirical optimization may be necessary.

​

Available objectives on the Zeiss LSM 780 are:

• ​N-Achroplan 10x/0.25

• Plan-Apochromat 20x/0.8

• C-Apochromat 40x/1.2 (water immersion)

• Plan-Apochromat 40x/1.4 (oil immersion)